|
Parameters
|
BCE-induced-DCs
|
Non-BCE-induced-DCs
|
|---|
|
CD11c (%)
|
16 ± 0.9
|
1.5 ± 0.01
|
|
MHCII (%)
|
13 ± 0.4
|
8 ± 0.3
|
|
IL4 (fold)
|
0.31 ± 0.01
|
0.61 ± 0.002
|
|
IL 10 (fold)
|
0.27 ± 0.03
|
0.73 ± 0.002
|
|
IL 12 (fold)
|
0.74 ± 0.005
|
0.2 ± 0.003
|
|
INF (fold)
|
1.3 ± 0.05
|
0.56 ± 0.002
|
|
IL 12 (Pg/ml)
|
4.5 ± 0.3
|
1.2 ± 0.1
|
|
INF-gamma (Pg/ml)
|
35 ± 3.2
|
22.5 ± 1.2
|
- The level of CD11c and MHCII were detected by FACSCalibur flow cytometer (BD Biosciences). cytokines expression (IL4, IL10, IL 12 and INF-gamma) was performed by RT-PCR. IL 12 and INF-gamma proteins levels were measured by ELISA (Komabiotech, Korea)
