Fig. 1

Effect of immunization with different subsets of DCs on surface marker expression in splenocytes of immunized mice. Splenocytes of immunized mice with BCE-induced-core pulsed dendritic cells, in a 3-dose immunization schedule at 4 weeks intervals, shows an elevated CD16⁺and MHCII⁺ populations when compared to RPMI - 1640 group. Spleens of immunized mice were dissociated by gentleMACS dissociator, as described in Materials and Methods, into a single-cell suspension. Freshly isolated splenocytes were stained with MHCII-FITC, Fcγ RIII/CD16-APC, and CD3-PE monoclonal antibodies and analyzed by flow cytometry. Data are shown as the percentage of splenocytes bearing surface antigen over total splenocytes population. * indicates a significant difference with RPMI group. Results are means ± SD of splenocytes of six mice. (PRPMI - 1640 group that received complete medium, nDCs group that received non-BCE-induced DC, nDCs-core group that received non-BCE-induced DCs transduced with core protein, iDCs group that received BCE-induced DCs, iDCs-core group that received BCE-induced DCs transduced with core protein)