Mechanisms of Heshouwuyin in regulating apoptosis of testicular cells in aging rats through mitochondrial pathway

Table 1 Primer sequences and annealing temperature of each gene (Table 1 should be listed at the end of 2.9 RNA isolation and qRT-PCR, on the Page 8)

Gene	Primer sequences	Annealing	Product
Gene	Primer sequences	Tm (°C)	size (bp)
Ccng1	F:CCTTCCAATTTCTGCAGCTC	60 °C	281
Ccng1	R:CTTGGAAACAAGCTCTTGCC	60 °C	281
GHR	F:ATCTTTGGCGGGTGTTCTTA	60 °C	78
GHR	R:TGTTGGCTATCTCGTAGTGGA	60 °C	78
Cabin1	F:AGTCCAGCAGAGCCAAGTCC	60 °C	313
Cabin1	R:TGAACCCGTCATACGTCCAT	60 °C	313
Capn8	F:ACGCTGTCTACCAGATTCCC	64 °C	342
Capn8	R:TGCCCACAAACTCCTCAAAC	64 °C	342
AK1	F:GTGGACGATAACGAGGAG	60 °C	166
AK1	R:TCAGGGAGTCAAGATAGGTG	60 °C	166
Cybrd1	F:CTTCGTACCATTCATTCCCACC	58 °C	171
Cybrd1	R:CCATTCCGTCTGCGTTGC	58 °C	171
Kdm2b	F:TGCCGAGATGAAATACCC	60 °C	170
Kdm2b	R:CATACAGAGCCAAGTTGTGC	60 °C	170
Glrx3	F:AGCACCCAAGTTAGAGGA	60 °C	292
Glrx3	R:TAGCAATTCACCGTTGTC	60 °C	292
ADAM5	F:CCGTTGAAATCTGGTCG	55 °C	107
ADAM5	R:AATGTGCTGCGGTCTAT	55 °C	107
BAX	F:GCGATGAACTGGACAACAACAT	62 °C	153
BAX	R:TAGCAAAGTAGAAAAGGGCAACC	62 °C	153
caspase-3	F:GACTGCGGTATTGAGACAGA	60 °C	209
caspase-3	R:CGAGTGAGGATGTGCATGAA	60 °C	209
DR6	F:CAGACCATGAACGAGCCT	60 °C	142
DR6	R:GTATCTTCCATCAGCCCAC	60 °C	142
14-3-3σ	R:TAGCTGGTGTAGCCCCACTT	55 °C	95
14-3-3σ	F:CATGGACATCAGCAAGAAGGA	55 °C	95
GSY2	F:CCTCGATGGCTGTGATTTCTGACAC	60 °C	172
GSY2	R:CTTGGGCGTTATCTCTGTGCAGCAA	60 °C	172
SOCS3	F:CTGGACCCATTCGGGAGTTC	62 °C	105
SOCS3	R:AACTGGGAGCTACCGACCATTG	62 °C	105
β-actin	F:GACGTTGACATCCGTAAAGACC	60 °C	115
β-actin	R:TGCTAGGAGCCAGGGCAGTA	60 °C	115

ISSN: 2662-7671