Fig. 12

Immunofluorescence and western blot for 14-3-3σ and statistical analysis. Immunofluorescence staining was used to detect the expression of 14-3-3σ and the rates of positive cells were quantified(I,III). The results showed that the rates of cells positive for 14-3-3σ were significantly lower in the NAG than in the YCG (P < 0.01). In the SWY1G and SWY2G, the rate of positive cells was significantly higher than in the NAG (P < 0.01). a: young control group (YCG); b: natural aging group (NAG); c: Heshouwuyin group 1 (SWY1G); d: Heshouwuyin group 2 (SWY2G). Western blot was used to detect the content of 14-3-3σ protein and statistical analysis was performed according to the gray level(II,IV). The results showed the variation trend was consistent with the results of immunofluorescence.1: natural aging group (NAG); 2: young control group (YCG); 3: Heshouwuyin group 1 (SWY1G); 4: Heshouwuyin group 2 (SWY2G). Each histogram represents the mean ± SD (n = 3). Columns with different letters represent significantly different values, while the same letters indicate no significant difference. Bars = 50 μm