Skip to main content
Fig. 11 | BMC Complementary and Alternative Medicine

Fig. 11

From: Mechanisms of Heshouwuyin in regulating apoptosis of testicular cells in aging rats through mitochondrial pathway

Fig. 11

Immunofluorescence and western blot for Cytc and statistical analysis. Immunofluorescence staining was used to detect the expression of Cytc and the rates of positive cells were quantified(I,III). The results showed that the rates of cells positive for Cytc were significantly higher in the NAG than in the YCG (P < 0.01). In the SWY1G and SWY2G, the rate of cells positive for Cytc was significantly lower than in the NAG (P < 0.01). a: young control group (YCG); b: natural aging group (NAG); c: Heshouwuyin group 1 (SWY1G); d: Heshouwuyin group 2 (SWY2G). Western blot was used to detect the content of Cytc protein and statistical analysis was performed according to the gray level(II,IV). The results showed the variation trend was consistent with the results of immunofluorescence.1: natural aging group (NAG); 2: young control group (YCG); 3: Heshouwuyin group 1 (SWY1G); 4: Heshouwuyin group 2 (SWY2G). Each histogram represents the mean ± SD (n = 3). Columns with different letters represent significantly different values, while the same letters indicate no significant difference. Bars = 50 μm

Back to article page