Fig. 1

β-Galactosidase staining and statistical analysis in different experimental groups. Cells positive for β-galactosidase stained blue. The β-galactosidase in all groups was distributed among the Leydig cells; in the NAG, the positive cells were also located among the spermatogonia. Statistical analysis showed that the β-galactosidase-positive rate was significantly higher in the NAG than YCG (P < 0.01), while the SWY1G and SWY2G showed a significantly lower rate (P < 0.01). a young control group (YCG); b: natural aging group (NAG); c: Heshouwuyin group 1 (SWY1G); d: Heshouwuyin group 2 (SWY2G). Each histogram represents the mean ± SD (n = 3). Columns with different letters represent significantly different values, while the same letters indicate no significant difference. Bar = 50 μm