Evaluation of Rumex hastatus D. Don for cytotoxic potential against HeLa and NIH/3T3 cell lines: chemical characterization of chloroform fraction and identification of bioactive compounds

Table 1 Cytotoxic activity of various samples of Rumex hastatus against HeLa and NIH/3T3 cell lines

Samples	Conc. (μg/ml)	HeLa Cell Line		NIH/3T3 Cell Line
Samples	Conc. (μg/ml)	Inhibition (%)	IC₅₀ (μg/ml)	Inhibition (%)	IC₅₀ (μg/ml)
Rh.Cr	500	63.25 ± 0.20***	347.33	74.96 ± 0.21***	174.52
	250	41.43 ± 1.15***		59.46 ± 0.54***
	125	29.00 ± 1.50***		43.07 ± 1.02***
	62.5	20.64 ± 1.60***		35.53 ± 0.61***
Rh.Hex	500	36.33 ± 3.50***	572.61	53.86 ± 0.85***	439.26
	250	15.46 ± 2.43***		40.60 ± 0.41***
	125	07.33 ± 0.68***		28.33 ± 0.33***
	62.5	05.03 ± 0.23***		21.50 ± 0.60***
Rh.Chf	500	81.50 ± 0.86***	151.52	82.13 ± 0.88***	53.37
	250	69.00 ± 2.80***		70.66 ± 0.49***
	125	43.66 ± 0.89***		64.02 ± 1.11***
	62.5	34.22 ± 0.23***		51.43 ± 0.61***
Rh.EtAc	500	79.66 ± 0.89***	166.50	72.76 ± 0.78***	158.73
	250	66.32 ± 1.30***		59.00 ± 0.57***
	125	40.93 ± 0.49***		46.86 ± 0.85***
	62.5	29.83 ± 1.36***		31.43 ± 0.81***
Rh.Aq	500	60.83 ± 1.36***	369.68	65.60 ± 0.41***	237.62
	250	42.53 ± 0.46***		51.96 ± 0.21***
	125	33.61 ± 1.70***		42.66 ± 0.49***
	62.5	21.33 ± 0.33***		36.13 ± 0.88***
Doxorubicin	500	96.63 ± 1.67	<0.1	98.53 ± 1.09	<0.1
	250	91.87 ± 0.25		93.76 ± 0.78
	125	89.46 ± 2.43		90.33 ± 0.88
	62.5	84.50 ± 0.86		87.46 ± 0.54

Data is represented as mean ± S.E.M; n = 3, ***: P < 0.001
Key: Rh.Cr Crude methanolic extract, Rh.Hex n-hexane fraction, Rh.Chf chloroform fraction, Rh.EtAc ethyl acetate fraction, Rh.Aq aqueous fraction

ISSN: 2662-7671