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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Bo-Gan-Whan regulates proliferation and migration of vascular smooth muscle cells

Fig. 4

Effect of Bo-Gan-Whan (BGH) on PDGF-BB induced phosphorylation of ERK1/2 and p38 MAPK in VSMCs. VSMCs were pretreated with or without (100, 250, and 500 μg/ mL) BGH for 1 h and then stimulated with 10 ng/mL of PDGF-BB for 15 min. For statistical analysis, densitometry of the band representing the phosphorylated form (p-ERK1/2 and p-p38) was normalized to the expression of their respective total forms (T-ERK1/2 and T-p38). Western blot analysis of phosphorylated ERK1/2 (P-ERK1/2) and p38 MAPK (p-p38) (a) and their corresponding relative intensities (b) and (c). Results are presented as the mean ± standard error of three independent experiments. Values with the same superscript letters are not significantly different as analyzed by Tukey’s multiple range test (P < 0.05)

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