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Table 3 Zone of inhibition (in mm) of the different concentrations of crude and solvent fractions of the leaves of Rhamnus prinoides against gram negative bacteria

From: Evaluation of the in vitro antibacterial activity of the solvent fractions of the leaves of Rhamnus prinoides L’Herit (Rhamnaceae) against pathogenic bacteria

Category of test

Concentration

Bacteria

P. aeruginosa

S. typhi

Stand

Clinic

stand

Crude

19.5 mg/well

11.00 ± 0.58a3c1d2

10.67 ± 0.88a3

13.33 ± 0.33a3d2

39 mg/well

13.33 ± 0.33a3

13.00 ± 0.58a3

14.00 ± 0.58a3d2

78 mg/well

14.33 ± 0.33 a3

14.00 ± 0.58a3

17.67 ± 0.67a3

Cip 0.005 mg/disc*

25.67 ± 0.33

24.33 ± 0.88

30.00 ± 0.58

Methanol

19.5 mg/well

10.33 ± 0.33a3d1

10.33 ± 0.33a3c1d2

11.00 ± 0.58a3d2

39 mg/well

12.00 ± 1.00a3

12.33 ± 0.33a3

12.67 ± 0.88a3d1

78 mg/well

13.67 ± 0.33a3

13.67 ± 0.33a3

15.67 ± 0.33a3

Cip 0.005 mg/disc*

24.33 ± 0.33

24.00 ± 0.58

31.00 ± 0.58

Chloroform

19.5 mg/well

---

---

10.33 ± 0.33a3d2e1

39 mg/well

---

---

11.67 ± 0.33a3

78 mg/well

---

---

13.00 ± 0.58a3g3

Cip 0.005 mg/disc*

NT

NT

30.33 ± 0.33

  1. Values are expressed as Mean ± S.E.M (n = 3), analysis was performed with One-Way ANOVA followed by Tukey test; acompared to positive control, bto 19.5 mg/well, cto 39 mg/well, dto 78 mg/well, eto crude 19.5 mg/well, fto crude 39 mg/well, gto crude 78 mg/well; 1P < 0.05, 2P < 0.01, 3P < 0.001. The negative control has shown no antibacterial activity * = positive control, Stand = standard (ATCC) strains whereas Clinic = clinically isolated strains, Cip = Ciprofloxacin, --- = no activity