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Table 2 Zone of inhibition (in mm) of the different concentrations of crude and solvent fractions of the leaves of Rhamnus prinoides against gram negative bacteria

From: Evaluation of the in vitro antibacterial activity of the solvent fractions of the leaves of Rhamnus prinoides L’Herit (Rhamnaceae) against pathogenic bacteria

Category of test Concentration Bacteria
S. flexneri E. coli
Stand Clinic Stand clinic
Crude 19.5 mg/well 12.00 ± 0.58a3d2 11.67 ± 0.33 a3d2 11.67 ± .88a3d1 11.33 ± 0.67a3d2
39 mg/well 14.00 ± 0.00a3d1 13.67 ± 0.33 a3d1 12.67 ± 0.33a3 12.67 ± 0.33a3
78 mg/well 17.00 ± 0.58a3 16.00 ± 0.58 a3 15.00 ± 0.00a3 14.50 ± 0.29 a3
Cef 0.03 mg/disc* NT NT 28.33 ± 0.88 27.00 ± 0.58
Cip 0.005 mg/disc* 26.33 ± 0.67 25.00 ± 0.58 NT NT
Methanol 19.5 mg/well 11.00 ± 0.00a3d2 10.67 ± 0.67a3d2 10.33 ± 0.33a3d3 9.00 ± 0.58a3c2d3
39 mg/well 13.00 ± 0.58a3 12.67 ± 0.33a3 12.00 ± 0.58a3d1 11.67 ± 0.33a3d2
78 mg/well 15.00 ± 0.58a3 14.67 ± 0.33a3 14.50 ± 0.29a3 14.33 ± 0.33a3
Cef 0.03 mg/disc* NT NT 27.67 ± 0.33 26.67 ± 0.33
Cip 0.005 mg/disc* 27.00 ± 0.58 26.33 ± 0.67 NT NT
Chloroform 19.5 mg/well 10.00 ± 0.58a3d2 10.33 ± 0.33a3 --- ---
39 mg/well 11.33 ± 0.33a3f1 11.67 ± 0.67a3 ---- ---
78 mg/well 13.33 ± 0.33 a3g2 12.33 ± 0.33a3g2 --- ---
Cip 0.005 mg/disc* 25.00 ± 0.58 24.67 ± 0.33 NT NT
  1. Values are expressed as Mean ± S.E.M (n = 3), analysis was performed with One-Way ANOVA followed by Tukey test acompared positive control, bto 19.5 mg/well, cto 39 mg/well, dto 78 mg/well, eto crude 19.5 mg/well, fto crude 39 mg/well, gto crude 78 mg/well; 1P < 0.05, 2P < 0.01, 3P < 0.001. The negative control has shown no antibacterial activity. * = positive controls, Stand = standard (ATCC) strains whereas Clinic. = clinically isolated strains, NT = not tested, Cef = Cefoxitin, Cip = Ciprofloxacin, --- = no activitiy