Fig. 5

PPHs reverse the effects of H₂O₂ on the mRNA expression of osteocalcin, Runx2, and osterix in MC3T3-E1 osteoblastic cells. Osteocalcin (a), Runx2 (b), and Osterix (c) expression levels in cells treated for 6 h with 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs were analyzed by RT-PCR. Representative bands are shown from three independent experiments. Quantitative analysis of RNA expression was also performed. ^* p < 0.05 versus cells treated with H₂O₂ alone. PPHs porcine placenta hydrolysates