Fig. 4

PPHs alleviate H₂O₂-mediated inhibition of ALP activity in MC3T3-E1 osteoblastic cells. a ALP mRNA levels in cells treated for 6 h with 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs were analyzed by RT-PCR. Representative bands are shown from three independent experiments. Quantitative analysis of RNA expression was also performed. b ALP activity levels were analyzed in cells treated with 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs (n = 3). ^* p < 0.05 versus cells treated with H₂O₂ alone. PPHs porcine placenta hydrolysates