Fig. 2

PPHs decrease the H₂O₂-induced ER stress response in MC3T3-E1 osteoblastic cells. a Cells were exposed to 400 μM H₂O₂ or 0.1 μM Tg and 0, 25, 50, or 100 μg/mL PPHs for 6 h. Immunoblotting was performed using the indicated antibodies. b Cells were exposed to 400 μM H₂O₂ in the presence or absence of 100 μg/mL PPHs for 0, 2, 4, or 6 h. Immunoblotting was performed using the indicated antibodies. Blots are representative of three independent experiments. ^* p < 0.05 versus cells treated with H₂O₂ alone (n = 3). PPHs porcine placenta hydrolysates, Tg thapsigargin