Fig. 4

Effects of PST® treatment on apoptosis. Flow cytometric analysis of hTSCs survival rate before a 1-h PST® treatment and then 10, 24 and 48 h post treatment (right panel), as compared to control cells (left panel), through double staining with Annexin V-FITC and PI. Early apoptotic cells (Annexin V-positive/PI-negative) are localized in the lower right region, late apoptotic and necrotic cells (Annexin V-positive/PI-positive) in the upper regions, and vital cells (double negative) in the lower left region