Fig. 5

Modulation of Wnt/β-catenin and apoptotic signaling proteins by RSV – GSE in colon CSCs with attenuated p53. β-catenin (a) and its downstream targets c-Myc (b) and cyclin D1 (c) were suppressed by RSV-GSE compared to sulindac. Pro-apoptotic proteins cleaved PARP (d) and cytochrome C (e) levels were elevated by RSV-GSE greater than that of control and sulindac. Colon CSCs were treated with RSV-GSE at 9 μM and 12.5 μg/mL, or sulindac at 12.5 μg/mL for 24 h, and cytosolic and nuclear cell lysates were analyzed. Actin and topoisomerase-2β (Topo II b) were used as loading controls for cytosolic and nuclear proteins respectively. C = Control; SU = Sulindac; RG = RSV-GSE. Values are in means ± S.E. Means that differ by a common letter (a, b, c, or x, y, z) differ p < 0.05