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Table 3 Cytotoxicity of plant extracts and doxorubicin towards sensitive and drug-resistant cancer cell lines and normal cells as determined by the resazurin assay after 72 h

From: Cytotoxicity of methanol extracts of 10 Cameroonian medicinal plants towards multi-factorial drug-resistant cancer cell lines

Cell lines

Samples, IC50 values in μg/mL and degrees of resistancea (in brackets)

Extracts

Doxorubicin

AAB

AAR

ACL

ACB

CEM/ADR5000

6.53 ± 0.77 (4.50)

6.52 ± 0.57 (6.65)

22.57 ± 2.17 (2.81)

34.87 ± 3.91 (2.77)

108.00 ± 7.92 (975.60)

MDA-MB-231-pcDNA

5.99 ± 0.3

3.79 ± 0.44

29.51 ± 3.08

25.54 ± 3.01

0.61 ± 0.15

MDA-MB-231-BCRP Degree of resistance

10.30 ± 1.22 (1.72)

8.00 ± 1.01 (2.11)

35.42 ± 3.35 (1.20)

33.60 ± 2.14 (1.32)

4.33 ± 0.26 (7.12)

HCT116 (p53+/+)

5.13 ± 0.64

4.40 ± 0.32

33.15 ± 2.87

29.07 ± 2.68

0.78 ± 0.16

HCT116 (p53−/−) Degree of resistance

9.46 ± 0.88 (1.84)

10.77 ± 0.79 (2.45)

>80 (>2.41)

>80 (2.75)

2.25 ± 0.04 (2.88)

U87MG

6.29 ± 0.40

8.86 ± 0.69

38.63 ± 3.32

>80

0.59 ± 0.08

U87MG.ΔEGFR Degree of resistance

2.71 ± 0.35 (0.43)

3.43 ± 0.48 (0.39)

32.12 ± 1.19 (0.83)

31.84 ± 2.36 (<0.40)

3.38 ± 0.32 (5.76)

HepG2 Degree of resistance

7.54 ± 0.55 (3.87)

6.84 ± 0.64 (4.26)

>80

>80

2.12 ± 0.52 (>37.74)

AML12

29.18 ± 3.03

29.14 ± 2.17

>80

>80

>80

  1. aThe degree of resistance was determined as the ratio of IC50 value in the resistant divided by the IC50 in the sensitive cell line; CEM/ADR5000, MDA-MB-231-BCRP, HCT116 (p53−/−), U87MG.ΔEGFR and AML12 were used as the corresponding resistant counterpart for CCRF-CEM (Table 1), MDA-MB-231-pcDNA, HCT116 (p53+/+), U87MG and HepG2 cells, respectively. The tested methanol extracts were from AAB Albizia adianthifolia bark, AAR Albizia adianthifolia roots, ACL Alchornea cordifolia leaves, ACB Alchornea cordifolia bark. In bold: significant cytotoxic effects. Leukemia CEM/ADR5000 cells were tested in RPMI 1640 medium while carcinoma cells tested using DMEM medium, both containing 10 % FBS and 1 % penicillin-streptomycin