Table 2 Primers for human wild-type AR mRNA expression vector and mutant AR-Asn160 expression vector
Primer | Sequence |
|---|---|
wild-type AR | HindIIIF:5’ CCCAAGCTTATGGCAAGCCGTCTCCTG 3’ XhoIR:5’ CCGCTCGAGTCAAAACTCTTCATGGAAGGG 3’ |
mutant AR-Asn160 | HindIIIF:5’ CCCATCCTGGGGTTGGGTACCGCTAAGGGTGGTGGTTCTGGAGGTG3’ XhoIR:5’ CTGCCCTGGAGGGGACTTAGCGGTACCTCCCTGGCTATTATCTGAGCTT 3’ |