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Table 1 Gene name and sequences of primers used in the multiplex panel

From: Clausenidin induces caspase-dependent apoptosis in colon cancer

Gene Name

Forward sequence

Reverse sequence

Bax

AGGTGACACTATAGAATAGCAAACTGGTGCTCAA

GTACGACTCACTATAGGGAAACCACCCTGGTCTTG

Bcl-2

AGGTGACACTATAGAATACTGTGGATGACTGAGTACCT

GTACGACTCACTATAGGGATCAGAGACAGCCAGGAG

Apaf-1

AGGTGACACTATAGAATACATACTCTTTCACCAGATCA

GTACGACTCACTATAGGGAACAAGTTCTGTTTTTGCTTT

Cyt c

AGGTGACACTATAGAATAGAGCGAGTTTGGTTGC

GTACGACTCACTATAGGGAAAATCTTCTTGCCTTTCTC

Caspase 3

AGGTGACACTATAGAATATGTAGAAGAGTTTCGTGAGT

GTACGACTCACTATAGGGAGAGTTTTCAGTGTTCTCCAT

Caspase 9

AGGTGACACTATAGAATAGCTGGTGGAAGAGCTG

GTACGACTCACTATAGGGACTCTAAGCAGGAGATGAACA

Ī²-actin

AGGTGACACTATAGAATAGATCATTGCTCCTCCTGAGC

GTACGACTCACTATAGGGAAAAGCCATGCCAATCTCATC

  1. The Ī²-actin gene was used for normalization. Reverse transcription (RT) and PCR were done according to manufacturerā€™s instructions; RT reaction was at 480 Ā°C for 1 min; 370 Ā°C for 5 min; 420 Ā°C for 60 min; 950 Ā°C for 5 min; then held at 40 Ā°C, while PCR was as follows: initial denaturation at 950 Ā°C for 10 min, followed by two-step cycles of 940 Ā°C for 30 s and 550 Ā°C for 30 s, ending in a single extension cycle of 680 Ā°C for 1 min. Bax: Bcl-2-associated X protein; Bcl-2: B cell lymphoma 2; Apaf-1: Apoptotic protease activating factor 1; Cyt c: cytochrome complex; Caspase 3: Cysteine aspartic acid protease 3; Caspase 9: Cysteine aspartic acid protease 9