|
Samples
|
Positive control (A)
|
Negative control (B)
|
Optical densityC at 560 nm concentration (μg/ml)
|
% Inhibition of hemolysis at 1000 (μg/ml)
|
|---|
| | | |
100
|
250
|
500
|
1000
| |
|---|
|
AHM
|
1.45 ± 0.02
|
0.11 ± 0.05
|
0.69 ± 0.01
|
0.85 ± 0.05
|
0.96 ± 0.05
|
1.15 ± 0.01
|
79.3c
|
|
AHH
|
1.45 ± 0.03
|
0.11 ± 0.05
|
0.65 ± 0.05
|
0.71 ± 0.05
|
0.88 ± 0.05
|
0.98 ± 0.05
|
67.6d
|
|
AHC
|
1.45 ± 0.05
|
0.11 ± 0.05
|
0.51 ± 0.05
|
0.63 ± 0.03
|
0.71 ± 0.05
|
0.87 ± 0.02
|
60.0e
|
|
AHE
|
1.45 ± 0.06
|
0.11 ± 0.05
|
0.72 ± 0.04
|
0.89 ± 0.09
|
1.14 ± 0.01
|
1.42 ± 0.02
|
97.9a
|
|
AHB
|
1.45 ± 0.04
|
0.11 ± 0.05
|
0.67 ± 0.05
|
0.81 ± 0.05
|
0.92 ± 0.05
|
1.29 ± 0.03
|
88.9b
|
|
AHA
|
1.45 ± 0.07
|
0.11 ± 0.05
|
0.49 ± 0.05
|
0.59 ± 0.05
|
0.64 ± 0.05
|
0.79 ± 0.05
|
54.5f
|
|
QR
|
1.45 ± 0.08
|
0.11 ± 0.05
|
0.72 ± 0.04
|
0.89 ± 0.09
|
1.14 ± 0.01
|
1.43 ± 0.02
|
98.6a
|
- Each value is represented as mean ± SEM (n = 3). Values in same column followed by different letter (a–f) are significantly different (p <0.05), A; phosphate buffer saline replaced extract/fraction as this treatment results in 0 % hemolysis, B; hydrogen peroxide replaced extract to serve as a negative control since this treatment results in 100 % hemolysis, c; lower values are associated with increased cell lysis. AHM A. hydaspica methanol extract, AHH A. hydaspica nhexane fraction, AHE A. hydaspica ethyl-acetate. (Completely Randomized AOV followed by Tukey HSD All-Pairwise Comparison Test)
