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Table 4 Anti-hemolytic activity of A. hydaspica methanol extract and its soluble fractions against H2O2 induced hemolysis

From: Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts

Samples Positive control (A) Negative control (B) Optical densityC at 560 nm concentration (μg/ml) % Inhibition of hemolysis at 1000 (μg/ml)
    100 250 500 1000  
AHM 1.45 ± 0.02 0.11 ± 0.05 0.69 ± 0.01 0.85 ± 0.05 0.96 ± 0.05 1.15 ± 0.01 79.3c
AHH 1.45 ± 0.03 0.11 ± 0.05 0.65 ± 0.05 0.71 ± 0.05 0.88 ± 0.05 0.98 ± 0.05 67.6d
AHC 1.45 ± 0.05 0.11 ± 0.05 0.51 ± 0.05 0.63 ± 0.03 0.71 ± 0.05 0.87 ± 0.02 60.0e
AHE 1.45 ± 0.06 0.11 ± 0.05 0.72 ± 0.04 0.89 ± 0.09 1.14 ± 0.01 1.42 ± 0.02 97.9a
AHB 1.45 ± 0.04 0.11 ± 0.05 0.67 ± 0.05 0.81 ± 0.05 0.92 ± 0.05 1.29 ± 0.03 88.9b
AHA 1.45 ± 0.07 0.11 ± 0.05 0.49 ± 0.05 0.59 ± 0.05 0.64 ± 0.05 0.79 ± 0.05 54.5f
QR 1.45 ± 0.08 0.11 ± 0.05 0.72 ± 0.04 0.89 ± 0.09 1.14 ± 0.01 1.43 ± 0.02 98.6a
  1. Each value is represented as mean ± SEM (n = 3). Values in same column followed by different letter (a–f) are significantly different (p <0.05), A; phosphate buffer saline replaced extract/fraction as this treatment results in 0 % hemolysis, B; hydrogen peroxide replaced extract to serve as a negative control since this treatment results in 100 % hemolysis, c; lower values are associated with increased cell lysis. AHM A. hydaspica methanol extract, AHH A. hydaspica nhexane fraction, AHE A. hydaspica ethyl-acetate. (Completely Randomized AOV followed by Tukey HSD All-Pairwise Comparison Test)