Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts

Table 4 Anti-hemolytic activity of A. hydaspica methanol extract and its soluble fractions against H₂O₂ induced hemolysis

Samples	Positive control (A)	Negative control (B)	Optical density^C at 560 nm concentration (μg/ml)				% Inhibition of hemolysis at 1000 (μg/ml)
			100	250	500	1000
AHM	1.45 ± 0.02	0.11 ± 0.05	0.69 ± 0.01	0.85 ± 0.05	0.96 ± 0.05	1.15 ± 0.01	79.3^c
AHH	1.45 ± 0.03	0.11 ± 0.05	0.65 ± 0.05	0.71 ± 0.05	0.88 ± 0.05	0.98 ± 0.05	67.6^d
AHC	1.45 ± 0.05	0.11 ± 0.05	0.51 ± 0.05	0.63 ± 0.03	0.71 ± 0.05	0.87 ± 0.02	60.0^e
AHE	1.45 ± 0.06	0.11 ± 0.05	0.72 ± 0.04	0.89 ± 0.09	1.14 ± 0.01	1.42 ± 0.02	97.9^a
AHB	1.45 ± 0.04	0.11 ± 0.05	0.67 ± 0.05	0.81 ± 0.05	0.92 ± 0.05	1.29 ± 0.03	88.9^b
AHA	1.45 ± 0.07	0.11 ± 0.05	0.49 ± 0.05	0.59 ± 0.05	0.64 ± 0.05	0.79 ± 0.05	54.5^f
QR	1.45 ± 0.08	0.11 ± 0.05	0.72 ± 0.04	0.89 ± 0.09	1.14 ± 0.01	1.43 ± 0.02	98.6^a

Each value is represented as mean ± SEM (n = 3). Values in same column followed by different letter (^a–f) are significantly different (p <0.05), A; phosphate buffer saline replaced extract/fraction as this treatment results in 0 % hemolysis, B; hydrogen peroxide replaced extract to serve as a negative control since this treatment results in 100 % hemolysis, ^c; lower values are associated with increased cell lysis. AHM A. hydaspica methanol extract, AHH A. hydaspica nhexane fraction, AHE A. hydaspica ethyl-acetate. (Completely Randomized AOV followed by Tukey HSD All-Pairwise Comparison Test)

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