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Table 3 EC50 values of different antioxidant activities of extract and derived fractions of A. hydaspica

From: Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts

EC50 values

Sample

DPPH radical

Superoxide radical

Hydroxyl radical

Hydrogen peroxide radical

ABTS radical

Iron chelating power

β-carotene bleaching inhibition

Total antioxidant index at 250 μg/ml

Lipid peroxidation inhibition

AHM

19.5 ± 0.43a

43.0 ± 0.37b

39.0 ± 0.44a

40.3 ± 0.26a

193 ± 0.26d

41.7 ± 0.34a

86.4 ± 0.62d

1.6 ± 0.69b

156.1 ± 0.76d

AHH

46.5 ± 0.55b

59.3 ± 0.36c

42.3 ± 0.29b

80.6 ± 0.43c

401 ± 0.23e

91.7 ± 0.17b

173.5 ± 0.85d

1.2 ± 0.35c

562.3 ± 0.55g

AHE

18.0 ± 0.45a

38.3 ± 0.40a

35.7 ± 0.40a

37.2 ± 0.36a

173 ± 0.11c

40.3 ± 0.21a

48.4 ± 0.55b

1.7 ± 0.61b

61.3 ± 0.52c

AHC

65.0 ± 0.36c

90.8 ± 0.55d

63.0 ± 0.09c

115.6 ± 1.51d

600 ± 0.26e

130.1 ± 0.17d

260.6 ± 1.05f

0.7 ± 0.73e

467.3 ± 0.60f

AHB

16.7 ± 0.43a

49.0 ± 0.41b

33.0 ± 0.16a

42.0 ± 0.11a

98.0 ± 0.12a

39.0 ± 0.26a

66.2 ± 0.72c

1.7 ± 0.41a

50.0 ± 0.61b

AHA

39.5 ± 0.51b

46.3 ± 0.52b

47.0 ± 0.12b

68.1 ± 0.36b

478 ± 0.12e

103.0 ± 0.12c

131.6 ± 1.15e

1.3 ± 0.14e

401.1 ± 0.12e

AA

15.0 ± 0.41a

-

-

35.0 ± 0.21a

61.0 ± 0.21b

-

-

1.8 ± 0.24b

-

GA

-

32.3 ± 0.28a

29.0 ± 0.16a

-

-

-

-

-

43.5 ± 0.86a

C

-

-

-

-

-

37.7 ± 0.3a

40.4 ± 1.01a

-

-

BHA

-

-

-

-

-

-

 

-

59.3 ± 0.98c

BHT

-

-

-

-

-

-

50.11 ± 0.59b

-

 
  1. Values are expressed as Mean ± SEM (n = 3). Means with superscripts with different letters in the column are significantly (P <0.05) different from each other. - indicate not determined, AHM A. hydaspica methanol extract, AHH n-hexane fraction, AHE ethyl acetate fraction, AHC chloroform fraction, AHB n-butanol fraction, AHA aqueous fraction, AA Ascorbic acid, GA Gallic acid, C Catechin, BHA butylated hydroxy anisole, BHT butylated hydroxy toluene. (Completely Randomized AOV followed by Tukey HSD All-Pairwise Compariso Test)