Fig. 3
Inhibitory effects of OMT on ALD-induced type I and III collagen secretion of cardiac fibroblasts. Cardiac fibroblasts were pre-treated with 7.57 × 10−4 mol/L [High] or 3.78 × 10−4 mol/L [Low] OMT or without OMT for 2 h, and then coincubated with ALD (1 × 10−8 mol/L) for 46 h. a ELISA assays of type I collagen content in cell lysis buffer; (b) type III collagen content in cell lysis buffer; (c) type I collagen content in cell supernatant; and (d) type III collagen content in cell supernatant. Results are means ± SEM of three independent experiments. (*P < 0.05, **P < 0.01 vs. control cells; # P < 0.05, ## P < 0.01 vs. ALD alone, n = 5)