Fig. 8
Influence on O2 consumption, motility and selected protein marker expression. a Mitochondrial O2 consumption (respiration) in MG-63 cells and primary osteoblasts (POB) during exposure to 25 μg/ml VSM or BORM in comparison to the control (which was set to 100 %) determined by the Bionas® 2500 analyzing system combined with the metabolic chip Bionas DisocveryTM SC1000 equipped with Clark-type oxygen sensors. Both BORM and VSM reduce the respiration rate dramatically (99 and 60 % reduction, respectively) in tumorigenic osteoblasts. b Effect of VSM (25, 50 μg/ml) on MG-63 migration behavior in comparison to the control treatment (DMSO) in a 72 h time period in a wound healing assay (raw data available in Additional file 7: Figure S7). Notably, exposure to VSM prevents migration of MG-63 cells so that the cell lawn cannot be closed. c Invasion assay of BORM and VSM treated MG-63 cells. Only VSM reduced the invasion capacity, significantly. Mean ± SD, n = 3, *P < 0.5, significantly different compared to control, unpaired t-test. d Western blot analysis of proliferation (PCNA) and apoptosis (Bcl-2) marker expression in VSM or BORM treated (concentration series 1–50 μg/ml) MG-63 cells in comparison to the control (c). The stain free image of the polyacrylamide gel functions as loading control