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Table 3 Regression equations, linearity, LOD, and LOQ of the 7 marker compounds

From: Chondroprotective and anti-inflammatory effects of ChondroT, a new complex herbal medication

Compound Linear range
(μg/mL)
Regression equationa Coefficient of determination LODb
(μg/mL)
LOQc
(μg/mL)
Chlorogenic acid 1.56–100.00 y = 30,420.94x – 42,881.66 0.9996 0.03 0.10
Berberine Cl 0.78–50.00 y = 81,886.13x + 16,338.31 0.9999 0.01 0.02
Nodakenin 0.78–50.00 y = 43,427.95x + 6,116.21 0.9999 0.02 0.06
Isoferulic acid 0.78–50.00 y = 44,822.70x – 7,991.61 1.0000 0.02 0.07
Oxypeucedanin hydrate 1.56–100.00 y = 26,221.17x + 5,076.06 1.0000 0.04 0.12
Decursin 0.78–50.00 y = 37,915.21x + 52,952.92 1.0000 0.04 0.12
Decursinol angelate 0.78–50.00 y = 28,967.64x + 29,015.53 1.0000 0.05 0.16
  1. ay: peak area (mAU) of compounds; x: concentration (μg/mL) of compounds
  2. bLOD = 3 × 3.3 × standard deviation of blank / slope of regression
  3. cLOQ = 10 × standard deviation of blank / slope of regression