Table 4 Human primer sets used to confirm mRNA expression
Gene | Primers | |
|---|---|---|
Forward | Reverse | |
IFN-β | 5′-CATCAACTATAAGCAGCTCCA-3′ | 5′-TTCAAGTGGAGAGCAGTTGAG-3′ |
Mx-1 | 5′-CCAAAGACACTTCCTCTC-3′ | 5′-CAGTGTGGTGGTTGTACT-3′ |
GBP-1 | 5′-AGAGATCACGGACTACAGAA-3′ | 5′-TCTGTGGACGTGTCATAGAT-3′ |
ISG-15 | 5′-GAGAGGCAGCGAACTCATCT-3′ | 5′-CTTCAGCTCTGACACCGACA-3′ |
ISG-56 | 5′AAGGCAGGCTGTCCGCTTA-3′ | 5′-TCCTGTCCTTCATCCTGAAGCT-3′ |
IL-8 | 5′-CTCTCTTGGCAGCCTTCCTGATT-3′ | 5′-AACTTCTCCACAACCCTCTGCAC-3′ |
IL-6 | 5′-CCACACAGACAGCCACTCACC-3′ | 5′-CTACATTTGCCGAAGAGCCCTC-3′ |
TNF-α | 5′-ATGAGCACTGAAAGCAT-3′ | 5′-TCGACGGGGAGTCGAACT-3′ |
β-actia | 5′-CCAACCGCGAGAAGATGACC-3′ | 5′-GATCTTCATGAGGTAGTCAGT-3′ |