Fig. 6

Chemical characterization of Cortex Phellodendri by HPLC analysis and effects of PA-6 on virus replication. a Chemical compounds in CP was analyzed by the reversed-phase HPLC. b RAW264.7 cells treated with media alone, 1.0 μg/ml CP, 10.0 μg/ml PA-6, or 1000 unit/ml recombinant mouse IFN-β at 12 h prior to infection with PR8-GFP (MOI = 1.0). GFP expression images were obtained at 12 and 24 hpi (200X magnification). c PR8-GFP titer was determined by standard plaque assay. d Secreted cytokines of murine IFN-β, IL-6 and TNF-α measured by ELISA. Tests were performed in triplicate. The data shows representative means ± SD. (*P < 0.05 indicates a significant difference between groups)