Fig. 5

Induction of IFN-β, IFN-related and antiviral genes by Cortex Phellodendri in vitro. RAW264.7 and HEK293T cells were treated with media alone, CP (1.0 μg/ml), or 1000 units/ml of rmIFN-β or rhIFN-β. Time-dependent changes in mRNA expression after treatment in a RAW264.7 and c HEK293T cells were confirmed by RT-PCR and qRT-PCR respectively. b RBI of IFN-β, IFN-α, TNF-α, IL-6, and IFN-related genes (Mx1, ISG15 and ISG56) were determined using the image analysis program and are shown as a bar graph. All the samples normalized using GAPDH. Error bars indicate the range of values obtained from two independent experiments