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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: Porcine placenta hydrolysates regulate calcium disturbance in MC3T3-E1 osteoblastic cells

Fig. 1

PPHs protect against Tg-induced apoptosis in MC3T3-E1 osteoblastic cells. a Hoechst 33342 staining was performed in cells treated with 0.1 μM Tg in the presence or absence of 100 μg/mL PPHs for 24 h. Arrows identify cells with condensed or fragmented nuclei characteristic of apoptosis. Apoptotic cells were quantified based on nuclear condensation or fragmentation (right). Caspase-12 (b) and −3 activities (c) were analyzed in MC3T3-E1 cells treated with 0.1 μM Tg in the presence or absence of 100 μg/mL PPHs for 0, 12, 24, 36, or 48 h. d Cells were treated with 0.1 μM Tg in the presence or absence of 100 μg/mL PPHs for 0, 12, 24, or 48 h. Immunoblotting was performed with antibodies against caspase-12, caspase-3, Bax, Bcl2, and β-actin. *p < 0.05, significantly different from the Tg-treated condition. Tg, thapsigargin; PPHs, porcine placenta hydrolysates

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