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Table 2 Effects of selected fractions and their combinations on hepatocyte viability, ALT leakage and MDA formation

From: Active chemical fractions of stem bark extract of Khaya grandifoliola C.DC and Entada africana Guill. et Perr. synergistically protect primary rat hepatocytes against paracetamol-induced damage

Groups Cell Viability (% of control) ALT Activity (UI/L) [MDA] (μM/5 × 105 hepatocytes)
Control 100.000 ± 2.600 27.830 ± 4.720 0.131 ± 0.032
paracetamol 51.520 ± 3.510** 78.660 ± 4.850** 0.517 ± 0.053**
paracetamol + silymarin (130 μg/ml) 95.200 ± 6.950* 55.330 ± 4.360* 0.282 ± 0.033*
paracetamol + EaF10 (130 μg/ml) 96.940 ± 6.350* 56.160 ± 2.510* 0.303 ± 0.045*
paracetamol + KgF25 (100 μg/ml) 96.540 ± 4.260* 55.330 ± 3.320* 0.307 ± 0.033*
paracetamol + (EaF10 130 μg/ml + KgF25 10 μg/ml) 96.850 ± 3.920* 55.500 ± 3.000* 0.290 ± 0.041*
paracetamol + (EaF10 130 μg/ml + KgF25 30 μg/ml) 99.620 ± 4.250* 54.33 ± 1.750* 0.277 ± 0.029*
paracetamol + (EaF10 130 μg/ml + KgF25 50 μg/ml) 101.050 ± 1.880* 45.330 ± 5.000* 0.264 ± 0.037*
paracetamol + (KgF25 100 μg/ml + EaF10 13 μg/ml) 97.360 ± 0.590* 54.660 ± 4.310* 0.299 ± 0.060*
paracetamol + (KgF25 100 μg/ml + EaF10 39 μg/ml) 99.760 ± 0.200* 47.500 ± 3.000* 0.273 ± 0.019*
paracetamol + KgF25 100 μg/ml + EaF10 65 μg/ml) 102.360 ± 2.290* 42.160 ± 2.080* 0.235 ± 0.029*
paracetamol + (KgF25 100 μg/ml + EaF10 130 μg/ml) 105.510 ± 2.400* (0.581) 37.330 ± 2.36* (0.945) 0.179 ± 0.013* (0.995)
  1. Values are mean ± SD from two independent experiments in triplicate. **P < 0.05 compared with control group; *P < 0.05 compared with paracetamol (para)-intoxicated group; EaF10: methylene chloride/methanol (90:10 v/v) fraction of E. africana; KgF25: methylene chloride/methanol (75:25 v/v) fraction of K. grandifoliola. Numbers in brackets indicate the coefficient of drug interaction (CDI)