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Table 1 Protocol of treatment of hepatocytes in synergistic studies

From: Active chemical fractions of stem bark extract of Khaya grandifoliola C.DC and Entada africana Guill. et Perr. synergistically protect primary rat hepatocytes against paracetamol-induced damage

Groups Volume of cell suspensions (μl) Volume of fractions (μl) Volume of Toxicant or vehicle (μl) Plant fraction/combination concentration tested
I 980 10 10 10 EC50 EaF10
II 980 10 10 10 EC50 KgF25
III 980 10 10 10 EC50 EaF10 + 1 EC50 KgF25
IV 980 10 10 10 EC50 EaF10 + 3 EC50 KgF25
V 980 10 10 10 EC50 EaF10 + 5 EC50 KgF25
VI 980 10 10 10 EC50 KgF25 + 1 EC50 EaF10
VII 980 10 10 10 EC50 KgF25 + 3 EC50 EaF10
VIII 980 10 10 10 EC50 KgF25 + 5 EC50 EaF10
IX 980 10 10 10 EC50 KgF25 + 10 EC50 EaF10
silymarin 980 10 10 10 EC50 silymarin
paracetamol 990 0 10 0
control 990 0 10 0
  1. EC50: half efficient concentration; KgF25: methylene chloride/methanol (75:25 v/v) fraction of K. grandifoliola; EaF10: methylene chloride/methanol (90:10 v/v) fraction of E. africana. EC50 values are: 13.47 ± 2.06, 10.30 ± 1.66 and 13.71 ± 3.87 μg/ml for EaF10, KgF25 and silymarin, respectively. 10 EC50 EaF10, 10 EC50 KgF25 and 10 EC50 sil mean the fractions EaF10, KgF25 and silymarin have been tested at the final concentration of 130 μg/ml (10 × 13 μg/ml = 130 μg/ml), 100 μg/ml (10 × 10 μg/ml = 100 μg/ml) and 130 μg/ml (10 × 13 μg/ml = 130 μg/ml), respectively