Fig. 3
The protective effects of HEPS on Aβ-induced toxicity in PC12 cells. a, (b) PC12 cells were treated with different concentrations of Aβ1–40 (0, 0.2, 0.4, 0.6, 0.8 and 1.2 μM) for 24 and 48 h and the toxicity was analyzed. c, (d) PC12 cells were treated with different concentrations (0, 25, 50, 100, 200 and 250 μg/mL) of HEPS followed by addition of 1.2 μM Aβ1–40 and incubation for 24 and 48 h. Cell viability measurements were used to evaluate cell death using the MTT assay. Serum-free RPMI-1640 medium is indicated as N−. All values are mean ± SD and used a one-way analysis of variance (ANOVA, n = 6). All columns were significantly different (p < 0.05)