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Table 1 Primers used for real-time RT-PCR analysis

From: Anti-inflammatory effects of Viola yedoensis and the application of cell extraction methods for investigating bioactive constituents in macrophages

Target gene Primer sequence
TNF-α F: 5’-TTCTGTCTACTGAACTTCGGGGTGATCGGTCC-3’
  R: 5’-GTATGAGATAGCAAATCGGCTGACGGTGTGGG-3’
IL-6 F: 5’-TCCAGTTGCCTTCTTGGGAC-3’
  R: 5’-GTGTAATTAAGCCTCCGACTTG-3’
IL-1β F: 5’-ATGGCAACTGTTCCTGAACTCAACT-3’
  R: 5’-CAGGACAGGTATAGATTCTTTCCTTT-3’
iNOS F: 5’-GGCAGCCTGTGAGACCTTTG-3’
  R: 5’-GCATTGGAAGTGAAGCGTTTC-3’
COX-2 F: 5’-TGAGTACCGCAAACGCTTCTC-3’
  R: 5’-TGGACGAGGTTTTTCCACCAG-3’
HO-1 F: 5’-TGAAGGAGGCCACCAAGGAGG-3’
  R: 5’-AGAGGTCACCCAGGTAGCGGG-3’
β-actin F: 5’-AGAGGGAAATCGTGCGTGAC-3’
  R: 5’-CAATAGTGATGACCTGGCCGT-3’
  1. F forward, R reverse