Fig. 3

Viability of HRT-18 and 23132/87 cells continuously cultured with FWGE. Incubation of 23132/87 and HRT-18 cells with 10 mg/ml FWGE was cytotoxic after 72 h of continuous culture for 23132/87 cells and after 120 h for HRT-18 cells. Cells were incubated in medium with 10 % (v/v) fetal calf serum and cell viability was determined by crystal violet staining at the culture times indicated. The dashed line indicates the relative initial cell count at the start of treatment. For this, the seeded cells were stained with crystal violet directly after their adherence and the absorbance was normalized to 100 %. By definition, a cytotoxic effect was a reduction in initial viable cell count >15 %, a cytostatic effect a change in initial cell count ±15 % and a delayed growth effect an increase in the initial cell count >15 %. Results are shown as mean ± standard deviation for three independent experiments, each performed in triplicate for each time point. ***P < 0.001 in comparison to 24 h