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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Mineral pitch induces apoptosis and inhibits proliferation via modulating reactive oxygen species in hepatic cancer cells

Fig. 5

Huh-7 cells were cultured with MP (0 and 100 μg/ml) along with either NAC or L-NAME. a After 24 h of incubation, the cell proliferation was estimated using MTT as described in Methods (n = 3; *p < 0.05 compared to control; # p < 0.05 compared to MP 100 μg/ml). b The cells were incubated with MP (100 μg/ml) along with NAC or L-NAME. Apoptosis induction was determined using Annexin V and PI exclusion assays All the experiments were performed in triplicates and repeated at least three times (n = 3; *p < 0.05)

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