Fig. 7From: Amomum cardamomum L. ethyl acetate fraction protects against carbon tetrachloride-induced liver injury via an antioxidant mechanism in ratsEffect of EAAC treatment on CYP2E1 mRNA levels in rat liver. Liver tissue was homogenized and lysed with Trisure to isolate mRNA. After cDNA synthesized, both conventional PCR and real-time PCR was conducted to investigate mRNA expression level. The result of conventional PCR (CYP2E1 and GAPDH) was illuminated by gel electrophoresis. ## shows statistically significnat difference at p < 0.01 from the untreated group. * shows statistically significant differences at p < 0.05 from the CCl4 group and ** at p < 0.01Back to article page