Fig. 1

Morphological analysis and growth curve of E. tirucalli latex treatment in Hep-2 cell line. Hep-2 cells were seeded in complete MEM 10 % at a concentration of 5×10⁴ per well (6 well plate). The cell morphology observed in (a) the control group, characterized by a monolayer of nucleated cells was not altered in (b) the E. tirucalli latex [25 μg/mL] treated group for 3 days. 400× magnification. The growth curve observed to Hep-2 cells treated with (c) E. tirucalli latex [0.25, 2.5, 25, 250 μg/ml] for 1, 3, 5 and 7 days and (d) ethanol (vehicle) and E. tirucalli latex [25 μg/ml] for 1, 3, 5 and 7 days. Assays were performed in triplicate. Graph x = time (hours) and y = number of cells × 10⁴. P values <0.05 were significant