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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Ginsenoside Rg1 protects starving H9c2 cells by dissociation of Bcl-2-Beclin1 complex

Fig. 2

Ginsenoside Rg1 induces autophagy in H9c2 cells under starvation conditions. a Representative live cell images of GFP in H9c2 cells transfected with GFP-LC3 for 48 h in the presence or absence of ginsenoside Rg1 under starvation. b, c Western blot analysis of endogenous LC3 and p62 in H9c2 cells after ginsenoside Rg1 treatment. Densitometric analysis was used to quantify the levels of LC3-II and p62 in H9c2 cells with and without ginsenoside Rg1 treatment. Values are expressed as the mean ± SD, n = 3, # p < 0.05, ## p < 0.01, starvation model group (30–240 min) versus starvation 0 min model group or *p < 0.05, ginsenoside Rg1 treatment group versus the corresponding time point of starvation. Trend changes of LC3-II and p62 in H9c2 cells with and without ginsenoside Rg1 treatment. d Effects of ginsenoside Rg1 on LC3 levels in H9c2 cells in the presence or absence of 5 μM CQ at 90 min after starvation by Western blot analysis. *p < 0.05, H9c2 cells in the presence of CQ and ginsenoside Rg1 group (CQ + G-Rg1) versus ginsenoside Rg1-only group (G-Rg1)

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