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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Rhodiola crenulata extract regulates hepatic glycogen and lipid metabolism via activation of the AMPK pathway

Fig. 4

Effects of LY294002 (LY) and compound C (CC) on glycogen synthesis and TG content in HepG2 cells. HepG2 cells were incubated in high-glucose medium (33 mM) for 16 h and then pretreated with RCE for 12 h or kinase inhibitors (20 μM LY294002 or 10 μM compound C) prior to RCE treatment (15 μg/mL) for 1 h. Glycogen synthesis (a) and TG content (b) were measured. Expression levels of p-ACC, SREBP1c, and p-GSK3ß were measured by western blotting (c). A quantitative analysis of the protein levels of p-ACC (d), SREBP1c (e), and p-GSK3ß (f) was conducted. The results represent the mean ± SEM (n = 3). p < 0.05, p < 0.01, p < 0.001 vs. the sample without RCE; #p < 0.05, ## p < 0.01, and ###p < 0.001 vs. the RCE-treated sample

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