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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Rhodiola crenulata extract regulates hepatic glycogen and lipid metabolism via activation of the AMPK pathway

Fig. 2

Effects of RCE on glycogen synthesis in HepG2 cells. HepG2 cells were cultured in 24-well plates and exposed to medium without serum for 24 h. The cells were then incubated with RCE (μg/mL) for 6 h or insulin (Ins, 100 nM) for 1 h before 14C (U)-d-glucose was added. The intensity of 14C in glycogen was analyzed (a). Expression levels of p-GSK3ß and p-GS were analyzed by western blotting (b). A quantitative analysis of the relative levels of p-GSK3ß (c) and p-GS (d) was conducted. The results represent the mean ± SEM (n = 3), p < 0.05, p < 0.01, p < 0.001 vs. the sample without RCE

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