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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms’ Tumor 1 expression in K562 cells

Fig. 6

Effect of mammea E/BB on WT1 mRNA stability. To investigate the effect of mammea E/BB on WT1 mRNA stability, K562 cells were treated with 4 μM actinomycin D (Act D) in the presence or absence of 3.5 μM mammea E/BB for 0, 3, 6, 9, and 12 h and WT1 mRNA was assayed by Taqman qRT-PCR as shown in a. The WT1 mRNA levels from three independent experiments were quantified and are shown in b. GAPDH was used as an internal control. Data are the mean value ± SEM of three independent experiments

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