Fig. 5

Effect of mammea E/BB on WT1 protein stability. To investigate the effect of mammea E/BB on protein stability, K562 cells were treated with 3.5 μM mammea E/BB in the presence or absence of 5 μM MG132 for 6 h and assessed by immunoblotting. a WT1 protein levels from three independent experiments were quantified and are shown in b. c The effect of 3.5 μM mammea E/BB on WT1 protein levels was examined in the presence or absence of 5 μM MG132 for 0, 3, 6, 12, and 24 h. The relative protein levels were analyzed with a scanning densitometer. The relation of WT1 protein levels to mammea E/BB and mammea E/BB + MG132 were compared to the vehicle control and the MG132 control, respectively. d, e WT1 protein levels of K562 cells after treated with 50 μg/ml cycloheximide (CHX) in the absence or presence of 3.5 μM mammea E/BB for 0, 3, 6, 12, and 24 h were quantified and are shown in f. The relative protein levels were analyzed with a scanning densitometer and compared to the CHX control. GAPDH was used as an internal control. Data are the mean value ± SEM of three independent experiments