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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms’ Tumor 1 expression in K562 cells

Fig. 3

Inhibitory effects of mammea E/BB on WT1 protein levels and total cell number in K562 cells. K562 cells were treated with various concentrations (0.125–4.5 μM) of mammea E/BB for 72 h. a WT1 protein levels were detected by Western blotting. GAPDH was used as a loading control. b The protein levels presented as the percentage of vehicle control (0.008 % DMSO alone without the mammea E/BB in the culture medium), analyzed with scanning densitometer. c The total survival and dead cell number of K562 cells after treatment with various concentrations of mammea E/BB, determined by the trypan blue exclusion method. Data are the mean value ± SEM of three independent experiments. Asterisks (*) denote values that were significantly different from the vehicle control (p < 0.05)

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