Skip to main content
Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms’ Tumor 1 expression in K562 cells

Fig. 2

Inhibitory effects of mammea E/BB on WT1 protein levels and total cell number in K562 cells. a WT1 protein levels in K562 cells treated with 3.5 μM mammea E/BB for 24, 48, and 72 h, detected by Western blotting. GAPDH was used as a loading control. b The protein levels presented as the percentage of vehicle control (0.006 % DMSO alone without the mammea E/BB in the culture medium), analyzed with the scanning densitometer. c The total survival and dead cell number of K562 cells after treatment at various times as determined by the trypan blue exclusion method. Data are the mean value ± SEM of three independent experiments. Asterisks (*) denote values that were significantly different from the vehicle control (p < 0.05)

Back to article page
\