Fig. 4

Assessment of mitochondrial membrane potential in HepG2 cells exposed to Verbesina encelioides (VE) extract for 24 h. The fluorescence of Rh123was measured using a flow cytometer on log scale with FL1 filter. Panel a is a representative FACS image decline in the Rh123 fluorescence as a function of Verbesina encelioides extract concentrations. Each histogram in panel b represents mean ± S.D. values of Rh123 fluorescence obtained from HepG2 cells exposed to varying concentrations of Verbesina encelioides extract. *p < 0.05, ** p < 0.01 versus control