Table 1 Nucleotide sequences of primers used in real-time PCR
Gene (accession no.) | Primes | Nucleotiede sequences5‘-3’ | Length (bp) | Temp (°C) |
|---|---|---|---|---|
LPL | Forward | CGCTCCATCCATCTCTTC | 57.3 | |
Reverse | GGCTCTGACCTTGTTGAT | 159 | 55.0 | |
CD36 | Forward | GGTCCTTACACATACAGAGT | 55.8 | |
Reverse | CCACAGCCAGATTGAGAA | 163 | 55.0 | |
GAPDH | Forward | TCAACGGCACAGTCAAG | 55.0 | |
Reverse | TACTCAGCACCAGCATCA | 116 | 55.0 |