Skip to main content
Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Fig. 5

Effect of down-regulation of survivin by the rat medicated sera containing WYHZTL in fibroblasts. a The mRNA levels of survivin were detected by RT-PCR after treated with the sera containing WYHZTL for 48 h. GAPDH was applied as an endogenous control and densitometric values were normalized by GAPDH. b The protein levels of survivin were detected by western blotting after treated with the sera containing WYHZTL for 48 h. β-actin was used as an endogenous control and densitometric values were normalized by β-actin. C represents control group, N represents XAV939 inhibitor group, X represents rat medicated sera containing WYHZTL group. Representative results are shown. Densitometric values are shown as mean ± SD. Left panels represent RT-PCR/Western blotting gel results, right panels represent bar graphs for relative mRNA and protein levels for survivin. *P < 0.05, **P < 0.01. The results are the mean ± SD of at least 5 independent experiments

Back to article page

BMC Complementary Medicine and Therapies

ISSN: 2662-7671

Contact us