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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Fig. 4

Effect of down-regulation of cyclin D1 by the rat medicated sera containing WYHZTL in fibroblasts. a The mRNA levels of cyclin D1 were detected by RT-PCR after treated with the sera containing WYHZTL for 24, 48 and 72 h. GAPDH was applied as an endogenous control and densitometric values were normalized by GAPDH. b The protein levels of cyclin D1 were detected by Western blotting after treated with the sera containing WYHZTL for 24, 48 and 72 h. β-actin was used as an endogenous control and densitometric values were normalized by β-actin. Representative results are shown. C represents control group, N represents XAV939 inhibitor group, X represents Rat Medicated Sera containing WYHZTL group. Densitometric values are shown as mean ± SD. Left panels represent RT-PCR/Western blotting gel results, right panels represent bar graphs for relative mRNA and protein levels for cyclin D1. *P < 0.05, **P < 0.01, compared with the control group. The results are the mean ± SD of at least 5 independent experiments

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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