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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Wenyang Huazhuo Tongluo formula, a Chinese herbal decoction, improves skin fibrosis by promoting apoptosis and inhibiting proliferation through down-regulation of survivin and cyclin D1 in systemic sclerosis

Fig. 2

Effect of Rat Medicated Sera containing WYHZTL on proliferation of fibroblasts. a Cell morphology observation: cells cultured in DMEM containing the sera were grown in the flask and viewed directly under the Nikon Phase Contrast Inverted Microscopy (200× original magnification; Ti-S type, Nikon Co., Japan). b The CCK-8 assay was performed to examine cell proliferation. The absorbance measured at 450 nm by using an enzyme immunoassay analyzer (Dynatech MR4100, USA) after incubated for 24, 48 and 72 h. The mean and standard deviation of 5 relative proliferation rates for each well were calculated. C represents control group, N represents XAV939 inhibitor group, X represents rat medicated sera containing WYHZTL group. **P < 0.01 compared with each group

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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