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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Kinsenoside inhibits the inflammatory mediator release in a type-II collagen induced arthritis mouse model by regulating the T cells responses

Fig. 6

The effect of kinsenoside on helper T-cell differentiation in Th1 or Th2 polarized conditions. Th0 cells (CD4+) were sorted by FACSAria for RT-PCR analysis. FACSAria (BD Biosciences) was used to sort Splenic CD4+ cellswith the purity of >95% (a). Gene expression was measured by RT-PCR for T-bet (b), GATA-3 (c) in polarized splenocytes and compared with non-stimulated splenocytes (day 7). RT-PCR data were normalized to GAPDH expression and presented as means ± SD, n = 3 in each group. ##P < 0.01as compared with the vehicle group

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