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Table 1 Names, accession number and primer sequences used in the study

From: Effects of phenolic-rich extracts of Clinacanthus nutans on high fat and high cholesterol diet-induced insulin resistance

Gene name

 

Primer sequence

(with universal tag)

 
  

Forward primer

Reverse primer

AdiponectinR2

NM_001037979

AGGTGACACTATAGAATACACTCCTGGAGAGAAGG

GTACGACTCACTATAGGGACTGAATGCTGAGTGATACAT

IRS

NM_017071

AGGTGACACTATAGAATAAGCTGGAGGAGTCTTCAT

GTACGACTCACTATAGGGAAAGGGATCTTCGCTTT

Pik3

NM_133399

AGGTGACACTATAGAATACAAGGATCTGACTTATTTCC

GTACGACTCACTATAGGGACTAACCATGCTGTTACCAA

LeptinR

NM_012596

AGGTGACACTATAGAATACAAAGTCCAGGATGACAC

GTACGACTCACTATAGGGACTTGGACAAACTCAGAATG

PPIA a

NM_017101

AGGTGACACTATAGAATATTCTGTAGCTCAGGAGAGCA

GTACGACTCACTATAGGGATTGAAGGGGAATGAGGAAAA

GAPDHa, b

NM_017008

A GGTGACACTATAGAATAATGACTCTACCCACGGCAAG

GTACGACTCACTATAGGGAAGCATCACCCCATTTGATGT

KanR c

   
  1. a House Keeping gene, b Normalized gene, c Internal control
  2. Reverse transcription (RT) and PCR were done according to manufacturer’s instructions; RT reaction was at 48 °C for 1 min; 37 °C for 5 min; 42 °C for 60 min; 95 °C for 5 min, then hold at 4 °C, while PCR was as follows: initial denaturation at 95 °C for 10 min, followed by two-step cycles of 94 °C for 30 s and 55 °C for 30 s, ending in a single extension cycle of 68 °C for 1 min