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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Herbal preparation (HemoHIM) enhanced functional maturation of bone marrow-derived dendritic cells mediated toll-like receptor 4

Fig. 6

HemoHIM-treated BMDCs induced the activation of allogeneic CD8+ T cells derived from C57BL/6 OT-1 mice. CD8+ T cells were co-cultured for 24 h with C57BL/6-derived BMDCs that were treated with LPS (1 μg/ml) or HemoHIM (100 μg/ml). a Flow cytometry of intracellular IFN-γ staining in CD8+ T cells. b Culture supernatants were harvested after 24 h, and cytokine levels were measured by ELISA. The results are representative of three experiments. *, p < 0.05, **, p < 0.01, and ***, p < 0.001 vs. OVA257-264-specific T cells co-cultured with untreated BMDCs. +, p < 0.05, ++, p < 0.01, and +++, p < 0.001 vs. OVA257-264-specific T cells co-cultured with OVA257-264-pulsed BMDCs. Not detection (N.D) showed less than 10 pg/ml of cytokine secretion in this experiment

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